Working procedures for sterile rooms:

（1）無菌室滅菌。每次使用前開啟紫外線燈照射30min以上，或在使用前30min，對內外室用5%石炭酸噴霧。

(1) Sterilization in a sterile room. Turn on the ultraviolet lamp to irradiate for more than 30min before each use, or use 5% carbolic acid spray for internal and external rooms 30min before use.

（2）用肥皂洗手后把所需器材搬入外室；在外室換上已滅菌的工作服、工作帽和工作鞋，戴好口罩，然后用2%煤酚皂液將手浸洗2分鐘左右。

(2) Wash hands with soap and move the necessary equipment into the outer room; Change into sterilized work clothes, hats, and shoes in the outer room, wear a mask, and then soak your hands in 2% kerosene soap solution for about 2 minutes.

（3）將各種需用物品搬進內室進行清點、就位，用5%石炭酸在工作臺面上方和操作員站位空間噴霧，返回外室，5～10min后再進內室工作。

(3) Move various articles needed into the inner room for counting and positioning, use 5% carbolic acid to spray above the work table and the operator's standing space, return to the outer room, and then enter the inner room for work after 5~10min.

（4）接種操作前，用70%的酒精棉球擦手；進行無菌操作時，動作要輕緩，盡量減少空氣波動和地面揚塵。

(4) Before the vaccination operation, wipe your hands with a 70% alcohol cotton ball; When performing aseptic operations, the movements should be gentle and gentle to minimize air fluctuations and ground dust.

（5）工作中應注意安全。如遇棉塞著火，用手緊握或用濕布包裹熄滅，為避免燃燒擴大切勿用嘴吹；如遇有菌培養物灑落或打碎有菌容器時，應用浸潤5%石炭酸的抹布包裹后，并用浸潤5%石炭酸的抹布擦拭臺面或地面，用酒精棉球擦手后再繼續操作。

(5) Pay attention to safety during work. If a cotton plug catches fire, grip it tightly with your hand or wrap it with a damp cloth to extinguish it. To avoid combustion expansion, do not blow it with your mouth; If bacterial culture is spilled or broken into containers, wrap them with a cloth soaked in 5% carbolic acid and wipe the table or ground with a cloth soaked in 5% carbolic acid. Use an alcohol cotton ball to wipe your hands before continuing the operation.

（6）工作結束，立即將臺面收拾干凈，將不應在無菌室存放的物品和廢棄物全部拿出無菌室后，對無菌室用5%石炭酸噴霧，或開紫外線燈照射30min。

(6) At the end of the work, clean up the table immediately, take out all the articles and wastes that should not be stored in the sterile room, and then use 5% carbolic acid spray to the sterile room, or turn on the ultraviolet light to illuminate for 30min.

無菌室的設置：

Setting up of sterile rooms:

根據經濟和科學的角度來講，無菌室設置的基本要求如下：

From an economic and scientific perspective, the basic requirements for setting up a sterile room are as follows:

（1）門應設在離工作臺遠的位置上，內間應當設拉門，以減少空氣的波動；外間的門好也用拉門，要設在距內間遠的位置上。

(1) The door should be located far from the workbench, and sliding doors should be installed inside to reduce air fluctuations; The exterior door should also be equipped with a sliding door, located far away from the interior.

（2）無菌室應有內、外兩間，內間是無菌室，外間是緩沖室。房間容積不宜過大，以便于空氣滅菌。內間面積2×2.5＝5m2，外間面積1×2＝2m2，高以2.5m以下為宜，都應有天花板。

(2) There should be two sterile rooms, one inside and the other outside. The inner room is a sterile room and the outer room is a buffer room. The room volume should not be too large to facilitate air sterilization. Interior area 2 × 2.5=5m2, outer space area 1 × 2=2m2, with a height of 2.5m or less, all should have ceilings.

（3）無菌室容積小而嚴密，使用一段時間后，室內溫度很高，故應設置通氣窗。通氣窗應設在內室進門處的頂棚上（即離工作臺遠的位置），為雙層結構，外層為百葉窗，內層可用抽板式窗扇。通氣窗可在內室使用后、滅菌前開啟，以流通空氣。有條件可安裝恒溫恒濕機。

(3) The sterile room has a small and tight volume, and after a period of use, the indoor temperature is very high, so ventilation windows should be installed. The ventilation window should be located on the ceiling at the entrance of the inner room (i.e. far from the workbench), which is a double-layer structure, with the outer layer being a louver, and the inner layer can be equipped with a panel type window sash. The ventilation window can be opened after indoor use and before sterilization to circulate air. If conditions permit, a constant temperature and humidity machine can be installed.

（4）在分隔內間與外間的墻壁或“隔扇”上，應開一個小窗，作接種過程中必要的內外傳遞物品的通道，以減少人員進出內間的次數，降低污染程度。小窗寬60cm、高40cm、厚30cm，內外都掛對拉的窗扇。

(4) A small window should be opened on the wall or "partition" separating the inner and outer compartments to serve as a necessary passage for internal and external transmission of items during the vaccination process, in order to reduce the number of people entering and exiting the inner compartment and reduce the level of pollution. The small window has a width of 60cm, a height of 40cm, and a thickness of 30cm, with opposite sashes hanging both inside and outside.

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